Figure 2

HPLC separation of soluble venom from different geographical areas: A 2-mg protein portion from each venom was separated into a C18 reverse-phase column eluted with a linear gradient from solution A (0.12% TFA in water) to 60% solution B (0.10% TFA in acetonitrile), run for 60 minutes: (A) venom of scorpions collected in Moa and La Poa; (B) venom collected in Limonar and El Chote; (C) venom collected in Farallones.