Fig. 1
Effect of Bjussu and Bpir crude venom on the hemolytic activity of the complement system. This figure depicts the concentration-dependent inhibitory effect of (a, c, and e) Bjussu and (b, d, and f) Bpir crude venoms on the hemolytic activity of the (a, b, e, and f) classical and (c and d) alternative pathways of the complement system. Panels a to d: Control represents normal human serum incubated with buffer alone. Data are expressed as the mean ± standard deviation of the t½ values obtained for each venom concentration, based on three (CS-CP/LP) or two (CS-AP) independent experiments assayed in triplicate. *p < 0.05, **p < 0.001, or ***p < 0.0001 vs. control. Panels e and f: Linear regression graph, where the X-values represent the amount of (e) Bjussu and (f) Bpir crude venom (in μg/mL) and the Y-values represent the mean percentages of hemolytic activity inhibition. The IC50 values were calculated from three independent experiments. Bjussu: Bothrops jararacussu; Bpir: Bothrops pirajai; t½: time required to lyse 50 % of erythrocytes