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Fig. 2 | Journal of Venomous Animals and Toxins including Tropical Diseases

Fig. 2

From: Immunomodulatory activity of Tityus serrulatus scorpion venom on human T lymphocytes

Fig. 2

Lymphocyte subsets and their expression levels of activation markers after TsV treatment. Peripheral blood mononuclear cells (2 × 105 cells) were cultured with Tityus serrulatus scorpion venom (TsV; 25, 50, and 100 μg/mL) or phytohemagglutinin (PHA; 2 μg/mL; positive control) for 24 h, at 37 °C, under 5 % CO2, and further stained with monoclonal antibodies to simultaneously detect CD3+CD4+ (a, b, c) and CD3+CD8+ (d, e, f) T lymphocyte subsets, and the expression of three activation markers: CD69 (a, d), CD25 (b, e), and HLA-DR (c, f). Control: untreated cells. Data are expressed as mean ± SEM of six experiments. ***p <0.001 vs. control

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