Fig. 4

Sequence alignment of the deduced amino acid sequence of Vespa tropica HAase with other allergen venom HAases. VesT2s was aligned with the known HAases; VesV2a and VesV2b (Vespula vulgaris; active and inactive forms), VesG2a and VesG2b (Vespula germanica; active and inactive forms), Vespa magnifica (VesMa2), DolM2 (Dolichovespula maculata), PolA2 (Polistes annularis), PolP2 (Polybia paulista) and Api_M2 (Apis mellifera). The shaded yellow alignment corresponds to conserved residues in HAase. The N-terminus was shown in the underlined amino acid sequence obtained by Edman sequencing. The catalytic residues (D and E letters) are indicated with the red stars (✩). The conserved cysteine positions among the HAase are indicated with blue triangles (△)