Fig. 2

Polyacrylamide gel electrophoresis and Western blots of horse F(ab’)2 antitoxins and anti-rabies immunoglobulins. Serum samples were subjected to SDS-PAGE (a and c) and Western blot analysis (b and d) under non-reducing (a and b) and reducing (c and d) conditions. Molecular mass standards were included in all the runs and the relative molecular mass (Mr) are shown. Gels (a and c) were stained with silver and Western blot assays (b and d) were revealed with rabbit anti-horse IgG labelled with alkaline phosphatase. Anti-Bot: Anti-botulinum AB; Anti-Diph: Anti-diphteric; Anti-Tet: Antitetanic; Anti-Rab: Anti-rabies; H: heavy chain; L: light chain; pdH: pepsin-digested heavy chain