Fig. 1

Separation by HPLC of the venom of Centruroides hirsutipalpus. The soluble part of scorpion venom (0.75 mg of protein) was passed through a reverse phase C18 column and separated with a linear gradient of a solvent A (0.12% trifluoroacetic acid in water) to 60% of solvent B (0.10% TFA in acetonitrile), for 60 min